The all-in-one UP.SIGHT automates the single-cell cloning process (single-cell isolating and dispensing into microwell plates, centrifuging, and high-throughput imaging) to deliver a time-, space- and cost-saving solution for academic and industry labs alike. Boasting a clonality rate of 99.669%, UP.SIGHT’s unique combination of imaging from the nozzle, fluid well, and well bottom provides more efficient, reliable, and reproducible evidence of viable, clonally derived cell lines for regulatory submission of mammalian-derived therapeutics.